dor_id: 60461
506.#.#.a: Público
590.#.#.d: Los artículos enviados a la revista "Veterinaria México OA", se juzgan por medio de un proceso de revisión por pares
510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT); Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex); Scientific Electronic Library Online (SciELO); Bibliografía Latinoamericana (Biblat); La Red de Revistas Científicas de América Latina y el Caribe, España y Portugal (Redalyc); Connecting research and researchers (ORCiD)
561.#.#.u: https://www.fmvz.unam.mx/
650.#.4.x: Biotecnología y Ciencias Agropecuarias
336.#.#.b: article
336.#.#.3: Artículo de Investigación
336.#.#.a: Artículo
351.#.#.6: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index
351.#.#.b: Veterinaria México OA
351.#.#.a: Artículos
harvesting_group: RevistasUNAM
270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx
590.#.#.c: Open Journal Systems (OJS)
270.#.#.d: MX
270.1.#.d: México
590.#.#.b: Concentrador
883.#.#.u: https://revistas.unam.mx/catalogo/
883.#.#.a: Revistas UNAM
590.#.#.a: Coordinación de Difusión Cultural
883.#.#.1: https://www.publicaciones.unam.mx/
883.#.#.q: Dirección General de Publicaciones y Fomento Editorial
850.#.#.a: Universidad Nacional Autónoma de México
856.4.0.u: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/424/502
100.1.#.a: Cisneros Prado, Jadiel Leonel; Montiel Palacios, Felipe; Canseco Sedano, Rodolfo; Merchant Larios, Horacio
524.#.#.a: Cisneros Prado, Jadiel Leonel, et al. (2017). Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity. Veterinaria México OA; Vol. 4 Núm. 2, 2017. Recuperado de https://repositorio.unam.mx/contenidos/60461
245.1.0.a: Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity
502.#.#.c: Universidad Nacional Autónoma de México
561.1.#.a: Facultad de Medicina Veterinaria y Zootecnia, UNAM
264.#.0.c: 2017
264.#.1.c: 2017-05-05
653.#.#.a: aseptic process; ovarian tissue; immature follicles; cattle
506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY 4.0 Internacional, https://creativecommons.org/licenses/by/4.0/legalcode.es, para un uso diferente consultar al responsable jurídico del repositorio por medio del correo electrónico vetmexicooa@gmail.com
884.#.#.k: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/424
001.#.#.#: 131.oai:ojs.pkp.sfu.ca:article/424
041.#.7.h: eng
520.3.#.a: Ovaries from slaughtered cows are the most abundant source of preantral immature follicles for in vitro studies including maturation and in vitro fertilization. integrity of immature follicles may be compromised by inadequate management during the collection and transport of the gonads, limiting the success of a correct evaluation. this study assessed the effect of aseptic and transport of bovine ovaries on the structural and ultrastructural integrity of immature follicles from the ovarian cortex. ovaries from 20 different crossbred cows (bos taurus × b. indicus) were randomly assigned to one of two aseptic and transport treatments (1) the ovaries were washed once with 70 % ethanol for 10 s, rinsed twice with normal saline solution (oh + nss), and then transported in nss + 0.05 % gentamicin sulphate; or (2) the ovaries were washed three times with phosphate buffered saline (pbs) and transported in pbs + 0.05 % gentamicin sulphate. samples of the ovarian cortex were obtained from each treatment group. half of samples of each treatment was processed for histologic evaluation of follicular morphology and developmental stage using haematoxylin & eosin, and the other half was processed to analyse the ultrastructure of immature follicles using transmission electronic microscopy. The rates of good-quality follicles were 36.95 % and 88.6 % for oh + nss and pbs, respectively. histological analysis showed higher number of abnormal follicles in oh + nss than in pbs treated ovaries (f1,12 = 99.64, p = 0.0001). The proportions of morphological alterations were not significantly different between treatments (χ21,7 = 7.72, p = 0.358). The ultrastructure of immature follicles from oh + nss ovaries showed cell damage, revealed by abundant cell detritus around both, the oocyte and follicular cells. however, the presence of alterations was barely evident in both the cytoplasm and the nucleus of the granulosa cells.figure 2. semi-fine cuts of immature follicles present in bovine ovarian cortex treated with pbs (a, b, c; viewed at 40x) or oh+nss (d, e, f; viewed at 40x).
773.1.#.t: Veterinaria México OA; Vol. 4 Núm. 2 (2017)
773.1.#.o: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index
022.#.#.a: ISSN electrónico: 2448-6760
310.#.#.a: Trimestral
264.#.1.b: Facultad de Medicina Veterinaria y Zootecnia, UNAM
doi: https://doi.org/10.21753/vmoa.4.2.424
harvesting_date: 2023-11-08 13:10:00.0
856.#.0.q: application/pdf
last_modified: 2024-03-19 14:00:00
license_url: https://creativecommons.org/licenses/by/4.0/legalcode.es
license_type: by
_deleted_conflicts: 2-ea18be646164c4dcf628be2c09abe2d9
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