dor_id: 60461

506.#.#.a: Público

590.#.#.d: Los artículos enviados a la revista "Veterinaria México OA", se juzgan por medio de un proceso de revisión por pares

510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT); Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex); Scientific Electronic Library Online (SciELO); Bibliografía Latinoamericana (Biblat); La Red de Revistas Científicas de América Latina y el Caribe, España y Portugal (Redalyc); Connecting research and researchers (ORCiD)

561.#.#.u: https://www.fmvz.unam.mx/

650.#.4.x: Biotecnología y Ciencias Agropecuarias

336.#.#.b: article

336.#.#.3: Artículo de Investigación

336.#.#.a: Artículo

351.#.#.6: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index

351.#.#.b: Veterinaria México OA

351.#.#.a: Artículos

harvesting_group: RevistasUNAM

270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

590.#.#.c: Open Journal Systems (OJS)

270.#.#.d: MX

270.1.#.d: México

590.#.#.b: Concentrador

883.#.#.u: https://revistas.unam.mx/catalogo/

883.#.#.a: Revistas UNAM

590.#.#.a: Coordinación de Difusión Cultural

883.#.#.1: https://www.publicaciones.unam.mx/

883.#.#.q: Dirección General de Publicaciones y Fomento Editorial

850.#.#.a: Universidad Nacional Autónoma de México

856.4.0.u: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/424/502

100.1.#.a: Cisneros Prado, Jadiel Leonel; Montiel Palacios, Felipe; Canseco Sedano, Rodolfo; Merchant Larios, Horacio

524.#.#.a: Cisneros Prado, Jadiel Leonel, et al. (2017). Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity. Veterinaria México OA; Vol. 4 Núm. 2, 2017. Recuperado de https://repositorio.unam.mx/contenidos/60461

245.1.0.a: Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Facultad de Medicina Veterinaria y Zootecnia, UNAM

264.#.0.c: 2017

264.#.1.c: 2017-05-05

653.#.#.a: aseptic process; ovarian tissue; immature follicles; cattle

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY 4.0 Internacional, https://creativecommons.org/licenses/by/4.0/legalcode.es, para un uso diferente consultar al responsable jurídico del repositorio por medio del correo electrónico vetmexicooa@gmail.com

884.#.#.k: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/424

001.#.#.#: 131.oai:ojs.pkp.sfu.ca:article/424

041.#.7.h: eng

520.3.#.a: Ovaries from slaughtered cows are the most abundant source of preantral immature follicles for in vitro studies including maturation and in vitro fertilization. integrity of immature follicles may be compromised by inadequate management during the collection and transport of the gonads, limiting the success of a correct evaluation. this study assessed the effect of aseptic and transport of bovine ovaries on the structural and ultrastructural integrity of immature follicles from the ovarian cortex. ovaries from 20 different crossbred cows (bos taurus × b. indicus) were randomly assigned to one of two aseptic and transport treatments (1) the ovaries were washed once with 70 % ethanol for 10 s, rinsed twice with normal saline solution (oh + nss), and then transported in nss + 0.05 % gentamicin sulphate; or (2) the ovaries were washed three times with phosphate buffered saline (pbs) and transported in pbs + 0.05 % gentamicin sulphate. samples of the ovarian cortex were obtained from each treatment group. half of samples of each treatment was processed for histologic evaluation of follicular morphology and developmental stage using haematoxylin & eosin, and the other half was processed to analyse the ultrastructure of immature follicles using transmission electronic microscopy. The rates of good-quality follicles were 36.95 % and 88.6 % for oh + nss and pbs, respectively. histological analysis showed higher number of abnormal follicles in oh + nss than in pbs treated ovaries (f1,12 = 99.64, p = 0.0001). The proportions of morphological alterations were not significantly different between treatments (χ21,7 = 7.72, p = 0.358). The ultrastructure of immature follicles from oh + nss ovaries showed cell damage, revealed by abundant cell detritus around both, the oocyte and follicular cells. however, the presence of alterations was barely evident in both the cytoplasm and the nucleus of the granulosa cells.figure 2. semi-fine cuts of immature follicles present in bovine ovarian cortex treated with pbs (a, b, c; viewed at 40x) or oh+nss (d, e, f; viewed at 40x).

773.1.#.t: Veterinaria México OA; Vol. 4 Núm. 2 (2017)

773.1.#.o: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index

022.#.#.a: ISSN electrónico: 2448-6760

310.#.#.a: Trimestral

264.#.1.b: Facultad de Medicina Veterinaria y Zootecnia, UNAM

doi: https://doi.org/10.21753/vmoa.4.2.424

harvesting_date: 2023-11-08 13:10:00.0

856.#.0.q: application/pdf

last_modified: 2024-03-19 14:00:00

license_url: https://creativecommons.org/licenses/by/4.0/legalcode.es

license_type: by

_deleted_conflicts: 2-ea18be646164c4dcf628be2c09abe2d9

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