Artículo

Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity

Facultad de Medicina Veterinaria y Zootecnia, UNAM, publicado en Veterinaria México OA, y cosechado de Revistas UNAM

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Procedencia del contenido

Entidad o dependencia
Facultad de Medicina Veterinaria y Zootecnia, UNAM
Revista
Repositorio
Contacto
Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

Cita

Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity. (2017). Veterinaria México OA; Vol 4, No 2, 2017. Recuperado de https://repositorio.unam.mx/contenidos/60461

Descripción del recurso

Colaborador(es)
Montiel Palacios, Felipe ; Canseco Sedano, Rodolfo ; Cisneros Prado, Jadiel Leonel ; Merchant Larios, Horacio
Afiliación del colaborador
Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana (UV), Veracruz, Veracruz, México.; Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana (UV), Veracruz, Veracruz, México.; Departamento de Biología Celular y Fisiología, Instituto de investigaciones Biomédicas, UNAM. Ciudad de México, México.; Facultad de Medicina Veterinaria y Zootecnia- UNAM.
Tipo
Artículo de Investigación
Área del conocimiento
Biotecnología y Ciencias Agropecuarias
Título
Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity
Fecha
2017-04-06
Resumen
Veterinaria México OA ISSN: 2448-6760 Cite this as: • Cisneros Prado JL, Montiel Palacios F, Canseco Sedano R, Merchant Larios H. Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity. Veterinaria México OA. 2017;4(2). doi: 10.21753/vmoa.4.2.424. Ovaries from slaughtered cows are the most abundant source of preantral immature follicles for in vitro studies including maturation and in vitro fertilization. Integrity of immature follicles may be compromised by inadequate management during the collection and transport of the gonads, limiting the success of a correct evaluation. This study assessed the effect of aseptic and transport of bovine ovaries on the structural and ultrastructural integrity of immature follicles from the ovarian cortex. Ovaries from 20 different crossbred cows (Bos taurus × B. indicus) were randomly assigned to one of two aseptic and transport treatments: (1) the ovaries were washed once with 70 % ethanol for 10 s, rinsed twice with normal saline solution (OH + NSS), and then transported in NSS + 0.05 % gentamicin sulphate; or (2) the ovaries were washed three times with phosphate buffered saline (PBS) and transported in PBS + 0.05 % gentamicin sulphate. Samples of the ovarian cortex were obtained from each treatment group. Half of samples of each treatment was processed for histologic evaluation of follicular morphology and developmental stage using haematoxylin & eosin, and the other half was processed to analyse the ultrastructure of immature follicles using transmission electronic microscopy. The rates of good-quality follicles were 36.95 % and 88.6 % for OH + NSS and PBS, respectively. Histological analysis showed higher number of abnormal follicles in OH + NSS than in PBS treated ovaries (F1,12 = 99.64, P = 0.0001). The proportions of morphological alterations were not significantly different between treatments (χ21,7 = 7.72, P = 0.358). The ultrastructure of immature follicles from OH + NSS ovaries showed cell damage, revealed by abundant cell detritus around both, the oocyte and follicular cells. However, the presence of alterations was barely evident in both the cytoplasm and the nucleus of the granulosa cells. Figure 2. Semi-fine cuts of immature follicles present in bovine ovarian cortex treated with PBS (A, B, C; viewed at 40X) or OH+NSS (D, E, F; viewed at 40X).
Tema
aseptic process; ovarian tissue; immature follicles; cattle
Idioma
eng
ISSN
2448-6760

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