dor_id: 43301

506.#.#.a: Público

590.#.#.d: si

510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT), Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex), Scientific Electronic Library Online (SciELO)

561.#.#.u: http://www.odonto.unam.mx/

561.#.#.a: Facultad de Odontología, UNAM

650.#.4.x: Medicina y Ciencias de la Salud

336.#.#.b: article

336.#.#.3: Artículo Técnico-Profesional

336.#.#.a: Artículo

351.#.#.6: http://revistas.unam.mx/index.php/rom

351.#.#.b: Revista Odontológica Mexicana

351.#.#.a: Artículos

harvesting_group: RevistasUNAM

270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

590.#.#.c: Open Journal Systems (OJS)

270.#.#.d: MX

270.1.#.d: México

590.#.#.b: Concentrador

883.#.#.u: http://www.revistas.unam.mx/front/

883.#.#.a: Revistas UNAM

590.#.#.a: Coordinación de Difusión Cultural, UNAM

883.#.#.1: https://www.publicaciones.unam.mx/

883.#.#.q: Dirección General de Publicaciones y Fomento Editorial, UNAM

850.#.#.a: Universidad Nacional Autónoma de México

856.4.0.u: http://revistas.unam.mx/index.php/rom/article/view/57725/51166

100.1.#.a: Cuin Macedo, Sergio Iván; Arroyo Cervantes, Roberto; Rodríguez Zaragoza, Diana Ened; Ruiz Reyes, Héctor; Calderón Rojas, Benigno Miguel

524.#.#.a: Cuin Macedo, Sergio Iván, et al. (2016). Proposal for experimental in vitro model to assess morphological alterations in erythrocytes exposed to 5.25% NaOCl. Revista Odontológica Mexicana; Vol 20, No 4-ING. Recuperado de https://repositorio.unam.mx/contenidos/43301

245.1.0.a: Proposal for experimental in vitro model to assess morphological alterations in erythrocytes exposed to 5.25% NaOCl

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Facultad de Odontología, UNAM

264.#.0.c: 2016

264.#.1.c: 2017-01-26

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC-ND 4.0 Internacional, https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es, fecha de asignación de la licencia 2017-01-26, para un uso diferente consultar al responsable jurídico del repositorio por medio de danyelacarmona@gmail.com

884.#.#.k: http://revistas.unam.mx/index.php/rom/article/view/57725

041.#.7.h: eng

520.3.#.a: Introduction: Sodium hypochlorite (NaOCl) is the chemical agent most frequently used as irrigation solution during endodontic therapy. When extruded to periapical tissue, it is highly toxic. In endodontics, hemolysis caused by NaCOl has been proven using different models, nevertheless, there is little or no evidence of morphological alterations in the cellular membrane of erythrocytes. Objective: To propose an experimental model which might allow to assess morphological alterations suffered by erythrocytes when they are exposed to NaOCl used in the dental practice by means of high resolution scanning electron microscopy (SEM). Materials and methods: In the present study, 20 mL of peripheral blood were obtained and deposited in tubes with EDTA (ethylenediaminetetraacetic acid) anticoagulant. Rinses were conducted with a phosphate buffer solution (Evan’s solution). Several dilutions of the erythrocyte sample were prepared (1:1, 1:2, 1:4, 1:8 and 1:16); 100 μL of each of these dilutions was obtained to be then confronted with 100 μL of dental use 5.25% NaOCl (Viarzoni-T, Medental®); 0.5 μL of these samples were taken to then be deposited in a sample holder made of Zn-Cu alloy which was subjected to a process of Cu ion metallization bath, following the old Spluttering method. Microphotographs were obtained with SEM. Results: Erythrocytes with alteration type anisocytosis and poikilocytosis (stomatocytes, elliptocytes and discocytes) were observed. Some structural characteristics of NaOCl crystals were equally observed. Conclusion: This experimental model allowed assessment of morphological changes experienced by erythrocytes when exposed to 5.25% NaOCl.

773.1.#.t: Revista Odontológica Mexicana; Vol 20, No 4-ING

022.#.#.a: ISSN: 1870-199X

264.#.1.b: Facultad de Odontología, UNAM

758.#.#.1: http://revistas.unam.mx/index.php/rom/index

handle: 00f691ccf6b2300e

harvesting_date: 2019-02-06 00:00:00.0

856.#.0.q: application/pdf

last_modified: 2022-11-11 19:00:00

license_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es

license_type: by-nc-nd

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