dor_id: 4119728

506.#.#.a: Público

590.#.#.d: Los artículos enviados a la revista "Veterinaria México OA", se juzgan por medio de un proceso de revisión por pares

510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT); Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex); Scientific Electronic Library Online (SciELO); Bibliografía Latinoamericana (Biblat); La Red de Revistas Científicas de América Latina y el Caribe, España y Portugal (Redalyc); Connecting research and researchers (ORCiD)

561.#.#.u: https://www.fmvz.unam.mx/

650.#.4.x: Biotecnología y Ciencias Agropecuarias

336.#.#.b: article

336.#.#.3: Artículo de Investigación

336.#.#.a: Artículo

351.#.#.6: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index

351.#.#.b: Veterinaria México OA

351.#.#.a: Artículos

harvesting_group: RevistasUNAM

270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

590.#.#.c: Open Journal Systems (OJS)

270.#.#.d: MX

270.1.#.d: México

590.#.#.b: Concentrador

883.#.#.u: https://revistas.unam.mx/catalogo/

883.#.#.a: Revistas UNAM

590.#.#.a: Coordinación de Difusión Cultural

883.#.#.1: https://www.publicaciones.unam.mx/

883.#.#.q: Dirección General de Publicaciones y Fomento Editorial

850.#.#.a: Universidad Nacional Autónoma de México

856.4.0.u: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/436/530

100.1.#.a: Bautista Garfias, Carlos Ramón; Rodríguez, Tania; Rojas, Carmen; Lira, José J.; Álvarez, Jesús A.; Polanco, Diego

524.#.#.a: Bautista Garfias, Carlos Ramón, et al. (2018). Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd. Veterinaria México OA; Vol. 5 Núm. 1, 2018. Recuperado de https://repositorio.unam.mx/contenidos/4119728

245.1.0.a: Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Facultad de Medicina Veterinaria y Zootecnia, UNAM

264.#.0.c: 2018

264.#.1.c: 2018-01-26

653.#.#.a: Anaplasma marginale; Stomoxys calcitrans; nested PCR

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY 4.0 Internacional, https://creativecommons.org/licenses/by/4.0/legalcode.es, para un uso diferente consultar al responsable jurídico del repositorio por medio del correo electrónico vetmexicooa@gmail.com

884.#.#.k: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/436

001.#.#.#: 131.oai:ojs.pkp.sfu.ca:article/436

041.#.7.h: eng

520.3.#.a: Data on the implication of the stable fly (stomoxys calcitrans, diptera mus­cidae), in the transmission of anaplasma marginale in tick-free cattle is scarce. hence, the objective of this investigation was to detect the presence of dna from a. marginale in stable flies caught near a bovine herd, which has been maintained free of ticks for 40 years, and occasionally presents some clinical cases of anaplasmosis. twenty-four batches of s. calcitrans (15 flies each) were collected in the morning and in the afternoon twice a week (except for one catch in one week of september and one catch in one week of december) during 12 catching days in a period of four months. their dna was obtained and analysed by nested pcr (npcr) to identify the presence of a. marginale dna. seven of the batches (29.16 %) were pos­itive for a. marginale, as detected by an npcr that targets the a. marginale msp5 gene. similarly, in two out of 12 catching days (16.66 %), those s. calcitrans batches collected in the morning and in the afternoon, were pos­itive for a. marginale, while those s. calcitrans groups collected in three out of 12 catching days (25 %), were positive for the rickettsia. The obtained re­sults suggest that a. marginale transmission is carried out mechanically by s. calcitrans, favouring the circulation and maintenance of the microorganism in this particular bovine herd.figure 1. a. example of a stable fly stomoxys calcitrans caught in this study (photograph carlos r. bautista).

773.1.#.t: Veterinaria México OA; Vol. 5 Núm. 1 (2018)

773.1.#.o: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index

022.#.#.a: ISSN electrónico: 2448-6760

310.#.#.a: Trimestral

264.#.1.b: Facultad de Medicina Veterinaria y Zootecnia, UNAM

doi: https://doi.org/10.21753/vmoa.5.1.436

harvesting_date: 2023-11-08 13:10:00.0

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file_creation_date: 2018-01-26 00:15:21.0

file_modification_date: 2018-01-26 18:20:05.0

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last_modified: 2024-03-19 14:00:00

license_url: https://creativecommons.org/licenses/by/4.0/legalcode.es

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Artículo

Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd

Bautista Garfias, Carlos Ramón; Rodríguez, Tania; Rojas, Carmen; Lira, José J.; Álvarez, Jesús A.; Polanco, Diego

Facultad de Medicina Veterinaria y Zootecnia, UNAM, publicado en Veterinaria México OA, y cosechado de Revistas UNAM

Licencia de uso

Procedencia del contenido

Entidad o dependencia
Facultad de Medicina Veterinaria y Zootecnia, UNAM
Revista
Repositorio
Contacto
Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

Cita

Bautista Garfias, Carlos Ramón, et al. (2018). Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd. Veterinaria México OA; Vol. 5 Núm. 1, 2018. Recuperado de https://repositorio.unam.mx/contenidos/4119728

Descripción del recurso

Autor(es)
Bautista Garfias, Carlos Ramón; Rodríguez, Tania; Rojas, Carmen; Lira, José J.; Álvarez, Jesús A.; Polanco, Diego
Tipo
Artículo de Investigación
Área del conocimiento
Biotecnología y Ciencias Agropecuarias
Título
Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd
Fecha
2018-01-26
Resumen
Data on the implication of the stable fly (stomoxys calcitrans, diptera mus­cidae), in the transmission of anaplasma marginale in tick-free cattle is scarce. hence, the objective of this investigation was to detect the presence of dna from a. marginale in stable flies caught near a bovine herd, which has been maintained free of ticks for 40 years, and occasionally presents some clinical cases of anaplasmosis. twenty-four batches of s. calcitrans (15 flies each) were collected in the morning and in the afternoon twice a week (except for one catch in one week of september and one catch in one week of december) during 12 catching days in a period of four months. their dna was obtained and analysed by nested pcr (npcr) to identify the presence of a. marginale dna. seven of the batches (29.16 %) were pos­itive for a. marginale, as detected by an npcr that targets the a. marginale msp5 gene. similarly, in two out of 12 catching days (16.66 %), those s. calcitrans batches collected in the morning and in the afternoon, were pos­itive for a. marginale, while those s. calcitrans groups collected in three out of 12 catching days (25 %), were positive for the rickettsia. The obtained re­sults suggest that a. marginale transmission is carried out mechanically by s. calcitrans, favouring the circulation and maintenance of the microorganism in this particular bovine herd.figure 1. a. example of a stable fly stomoxys calcitrans caught in this study (photograph carlos r. bautista).
Tema
Anaplasma marginale; Stomoxys calcitrans; nested PCR
Idioma
eng
ISSN
ISSN electrónico: 2448-6760

Enlaces