dor_id: 43346

506.#.#.a: Público

590.#.#.d: si

510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT), Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex), Scientific Electronic Library Online (SciELO)

561.#.#.u: http://www.odonto.unam.mx/

561.#.#.a: Facultad de Odontología, UNAM

650.#.4.x: Medicina y Ciencias de la Salud

336.#.#.b: article

336.#.#.3: Artículo Técnico-Profesional

336.#.#.a: Artículo

351.#.#.6: http://revistas.unam.mx/index.php/rom

351.#.#.b: Revista Odontológica Mexicana

351.#.#.a: Artículos

harvesting_group: RevistasUNAM

270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

590.#.#.c: Open Journal Systems (OJS)

270.#.#.d: MX

270.1.#.d: México

590.#.#.b: Concentrador

883.#.#.u: http://www.revistas.unam.mx/front/

883.#.#.a: Revistas UNAM

590.#.#.a: Coordinación de Difusión Cultural, UNAM

883.#.#.1: https://www.publicaciones.unam.mx/

883.#.#.q: Dirección General de Publicaciones y Fomento Editorial, UNAM

850.#.#.a: Universidad Nacional Autónoma de México

856.4.0.u: http://revistas.unam.mx/index.php/rom/article/view/63050/55428

100.1.#.a: Suárez Causado, Amileth; Madera Anaya, Meisser Vidal

524.#.#.a: Suárez Causado, Amileth, et al. (2017). Evaluation of two RNA extraction methods in children’s saliva. Revista Odontológica Mexicana; Vol 21, No 4 Ing. Recuperado de https://repositorio.unam.mx/contenidos/43346

245.1.0.a: Evaluation of two RNA extraction methods in children’s saliva

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Facultad de Odontología, UNAM

264.#.0.c: 2017

264.#.1.c: 2018-01-10

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC-ND 4.0 Internacional, https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es, fecha de asignación de la licencia 2018-01-10, para un uso diferente consultar al responsable jurídico del repositorio por medio de danyelacarmona@gmail.com

884.#.#.k: http://revistas.unam.mx/index.php/rom/article/view/63050

041.#.7.h: eng

520.3.#.a: Objective: To standardize an RNA (ribonucleic acid) extraction protocol in children’s saliva specimens. Methods: The study was conducted on saliva specimens from 60 children who participated in the study with their parents’ authorization. Comparison of two RNA extraction methods was established; methods assessed concentration, quality and yield. Moreover, genic expression of GUSB gene was achieved with RT-PCR (reverse transcriptasepolymerase chain reaction). Data were analyzed through measurements of central tendency and dispersion, frequencies and percentages. T-Student and χ2 tests were used in order to differentiate between both methods (p < 0.05). Results: Analysis of cell amounts per saliva ml revealed a mean of 564,977.8 (SD = 246,678.6); a RNA in children’s saliva extraction method was standardized, specifically the method using RNeasy® Protect Saliva Mini Kit Qiagen exhibited better characteristics of RNA concentration (p = 0.0000) and yield (p = 0.0000) when compared to the method using QIAzol®; no RNA (p = 0.146) quality differences were found. Conclusions: RNA could be extracted from children’s saliva specimens, therefore, it is suggested to use saliva for the molecular analysis of different oral and systemic diseases.

773.1.#.t: Revista Odontológica Mexicana; Vol 21, No 4 Ing

022.#.#.a: ISSN: 1870-199X

264.#.1.b: Facultad de Odontología, UNAM

758.#.#.1: http://revistas.unam.mx/index.php/rom/index

handle: 6de112bbc9c83be2

harvesting_date: 2019-02-06 00:00:00.0

856.#.0.q: application/pdf

last_modified: 2022-11-11 19:00:00

license_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es

license_type: by-nc-nd

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