Artículo

Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models

Facultad de Medicina Veterinaria y Zootecnia, UNAM, publicado en Veterinaria México OA, y cosechado de Revistas UNAM

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Procedencia del contenido

Entidad o dependencia
Facultad de Medicina Veterinaria y Zootecnia, UNAM
Revista
Repositorio
Contacto
Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

Cita

Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models. (2016). Veterinaria México OA; Vol 3, No 4, 2016. Recuperado de https://repositorio.unam.mx/contenidos/60415

Descripción del recurso

Colaborador(es)
Palomares-resendiz, Erika Gabriela ; Diaz-aparicio, Efren ; Arellano-reynoso, Beatriz ; Hernández-castro, Rigoberto ; Ibarra-zazueta, Cristina
Afiliación del colaborador
Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán 04510, Ciudad de México, México.; Departamento de Ecología y agentes patógenos, Hospital General Dr. Manuel Gea González, Tlalpan, 14080, Ciudad de México, México; CENID Microbiología Animal INIFAP, Cuajimalpa, 05110, Ciudad de México, México.
Tipo
Artículo de Investigación
Área del conocimiento
Biotecnología y Ciencias Agropecuarias
Título
Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models
Fecha
2016-12-09
Resumen
Veterinaria México OA ISSN: 2448-6760 Cite this as: • Ibarra Zazueta C, Arellano Reynoso B, Hernández Castro R, Palomares Resendiz EG, Diaz Aparicio E. Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models. Veterinaria México OA. 2016;3(4). doi: 10.21753/vmoa.3.4.366 Caseous lymphadenitis of small ruminants causes economic losses worldwide. To date, no effective vaccine has been developed against the causative agent of this disease, Corynebacterium pseudotuberculosis. The objective of the present work was to evaluate an aroA mutant gene strain of C. pseudotuberculosis in cellular and murine models, for attenuation and the ability to stimulate an immune response. The intracellular survival of the aroA mutant strain and the wild type strain (WT) of C. pseudotuberculosis was evaluated in J774A.1 murine macrophages using a multiplicity of infection (MOI) of 1:1 with the following infection times: 30 min, and 1, 2, 4, 8, 12, and 24 h. The largest difference in the intracellular survival of the mutant was observed 30 min post-infection. After subcutaneous skin vaccination, the subcutaneous lesion progression observed on the 14th day was more severe in those animals that were vaccinated with the WT strain. An analysis of the residual virulence in the murine model did not reveal any bacteria in mice vaccinated with the aroA strain on day 28 post-vaccination. Mice vaccinated with the mutant showed 50 % protection against the intraperitoneal challenge, exceeding that of the control group (41.67 %). We conclude that the virulence of the aroA mutant was significantly attenuated in both cellular and murine models according to the residual virulence detected in mice. However, vaccination with the mutant failed to confer at least 80 % protection, which is desirable for an immunogen. Hence, this study contributes to the knowledge of the immune response against Corynebacterium pseudotuberculosis.
Tema
Corynebacterium pseudotuberculosis; aroA gene; vaccination; cellular model; murine model
Idioma
eng
ISSN
2448-6760

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