dor_id: 4149593

506.#.#.a: Público

590.#.#.d: Los artículos enviados a la revista "Veterinaria México OA", se juzgan por medio de un proceso de revisión por pares

510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT); Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex); Scientific Electronic Library Online (SciELO); Bibliografía Latinoamericana (Biblat); La Red de Revistas Científicas de América Latina y el Caribe, España y Portugal (Redalyc); Connecting research and researchers (ORCiD)

561.#.#.u: https://www.fmvz.unam.mx/

650.#.4.x: Biotecnología y Ciencias Agropecuarias

336.#.#.b: article

336.#.#.3: Artículo de Investigación

336.#.#.a: Artículo

351.#.#.6: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index

351.#.#.b: Veterinaria México OA

351.#.#.a: Artículos

harvesting_group: RevistasUNAM

270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

590.#.#.c: Open Journal Systems (OJS)

270.#.#.d: MX

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883.#.#.u: https://revistas.unam.mx/catalogo/

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883.#.#.1: https://www.publicaciones.unam.mx/

883.#.#.q: Dirección General de Publicaciones y Fomento Editorial

850.#.#.a: Universidad Nacional Autónoma de México

856.4.0.u: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/1106/943

100.1.#.a: Alvarado Fernández, Juan Carlos; Salas Rojas, Carolina; Estrella Morales, Josimar; González, Rocío; Cordero Solorzano, Juan Miguel; Aguilar Arguedas, Olga; Soto Araya, Yinnel; Perez Villalobos, Diana; Leon, Bernal

524.#.#.a: Alvarado Fernández, Juan Carlos, et al. (2023). Evaluation of a rapid immunochromatographic assay for the diagnosis of rabies in regional laboratories of Costa Rica. Veterinaria México OA; Vol. 10, 2023. Recuperado de https://repositorio.unam.mx/contenidos/4149593

245.1.0.a: Evaluation of a rapid immunochromatographic assay for the diagnosis of rabies in regional laboratories of Costa Rica

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Facultad de Medicina Veterinaria y Zootecnia, UNAM

264.#.0.c: 2023

264.#.1.c: 2023-10-23

653.#.#.a: Costa Rica; Rabies; rapid immunochromatographic diagnostic test (RIDT); direct fluorescent antibody test (FAT); comparison; Diagnosis

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY 4.0 Internacional, https://creativecommons.org/licenses/by/4.0/legalcode.es, para un uso diferente consultar al responsable jurídico del repositorio por medio del correo electrónico vetmexicooa@gmail.com

884.#.#.k: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/1106

001.#.#.#: 131.oai:ojs.pkp.sfu.ca:article/1106

041.#.7.h: eng

520.3.#.a: Rabies is a viral, lethal, and zoonotic disease with worldwide distribution, primarily transmitted through bites from infected dogs and bats. The biosafety laboratory (lse-lanaseve) of the animal health service of costa rica (senasa) serves as the national reference laboratory for diagnosing rabies in humans and animals. since regional laboratories lack the equipment for the direct fluorescent antibody test (fat), we evaluated the rapid immunochromatographic diagnostic test (ridt) from bionote, employing fat as a reference, to improve rabies diagnosis. we analyzed 193 brain tissue samples between 2014 and 2019. out of these, 174 came from species that ridt has been validated for bovines (162), dogs (10), and raccoons (2). The rest were from unvalidated species, including horses (7), humans (1), and others. among the 174 validated samples, 26 bovine samples were positive for both ridt and fat. reviewing all 193 samples, 28 were positive and 165 negative using both methods. two horse samples presented inconsistencies, being positive on fat but negative on ridt; these were subsequently verified as false negatives by rt-pcr. ridt exhibited a sensitivity of 94 % (ci95, 83.9-102.3), specificity of 100 %, positive predictive value (ppv) of 100 %, and negative predictive value (npv) of 99 % (ci95, 97.1-100.5). ridt has demonstrated reliability in quickly diagnosing rabies for validated species. we advise its application in senasa"s regional laboratories for those particular species. if there"s uncertainty, samples should be sent to lse-lanaseve for fat or rt-pcr confirmation.

773.1.#.t: Veterinaria México OA; Vol. 10 (2023)

773.1.#.o: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index

022.#.#.a: ISSN electrónico: 2448-6760

310.#.#.a: Trimestral

264.#.1.b: Facultad de Medicina Veterinaria y Zootecnia, UNAM

doi: https://doi.org/10.22201/fmvz.24486760e.2023.1106

harvesting_date: 2023-11-08 13:10:00.0

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last_modified: 2024-03-19 14:00:00

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Artículo

Evaluation of a rapid immunochromatographic assay for the diagnosis of rabies in regional laboratories of Costa Rica

Alvarado Fernández, Juan Carlos; Salas Rojas, Carolina; Estrella Morales, Josimar; González, Rocío; Cordero Solorzano, Juan Miguel; Aguilar Arguedas, Olga; Soto Araya, Yinnel; Perez Villalobos, Diana; Leon, Bernal

Facultad de Medicina Veterinaria y Zootecnia, UNAM, publicado en Veterinaria México OA, y cosechado de Revistas UNAM

Licencia de uso

Procedencia del contenido

Entidad o dependencia
Facultad de Medicina Veterinaria y Zootecnia, UNAM
Revista
Repositorio
Contacto
Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

Cita

Alvarado Fernández, Juan Carlos, et al. (2023). Evaluation of a rapid immunochromatographic assay for the diagnosis of rabies in regional laboratories of Costa Rica. Veterinaria México OA; Vol. 10, 2023. Recuperado de https://repositorio.unam.mx/contenidos/4149593

Descripción del recurso

Autor(es)
Alvarado Fernández, Juan Carlos; Salas Rojas, Carolina; Estrella Morales, Josimar; González, Rocío; Cordero Solorzano, Juan Miguel; Aguilar Arguedas, Olga; Soto Araya, Yinnel; Perez Villalobos, Diana; Leon, Bernal
Tipo
Artículo de Investigación
Área del conocimiento
Biotecnología y Ciencias Agropecuarias
Título
Evaluation of a rapid immunochromatographic assay for the diagnosis of rabies in regional laboratories of Costa Rica
Fecha
2023-10-23
Resumen
Rabies is a viral, lethal, and zoonotic disease with worldwide distribution, primarily transmitted through bites from infected dogs and bats. The biosafety laboratory (lse-lanaseve) of the animal health service of costa rica (senasa) serves as the national reference laboratory for diagnosing rabies in humans and animals. since regional laboratories lack the equipment for the direct fluorescent antibody test (fat), we evaluated the rapid immunochromatographic diagnostic test (ridt) from bionote, employing fat as a reference, to improve rabies diagnosis. we analyzed 193 brain tissue samples between 2014 and 2019. out of these, 174 came from species that ridt has been validated for bovines (162), dogs (10), and raccoons (2). The rest were from unvalidated species, including horses (7), humans (1), and others. among the 174 validated samples, 26 bovine samples were positive for both ridt and fat. reviewing all 193 samples, 28 were positive and 165 negative using both methods. two horse samples presented inconsistencies, being positive on fat but negative on ridt; these were subsequently verified as false negatives by rt-pcr. ridt exhibited a sensitivity of 94 % (ci95, 83.9-102.3), specificity of 100 %, positive predictive value (ppv) of 100 %, and negative predictive value (npv) of 99 % (ci95, 97.1-100.5). ridt has demonstrated reliability in quickly diagnosing rabies for validated species. we advise its application in senasa"s regional laboratories for those particular species. if there"s uncertainty, samples should be sent to lse-lanaseve for fat or rt-pcr confirmation.
Tema
Costa Rica; Rabies; rapid immunochromatographic diagnostic test (RIDT); direct fluorescent antibody test (FAT); comparison; Diagnosis
Idioma
eng
ISSN
ISSN electrónico: 2448-6760

Enlaces