Artículo

Mycobacterium avium subsp. paratuberculosis down-regulates mRNA expression of iron-induced macrophage Ferroportin 1

Facultad de Medicina Veterinaria y Zootecnia, UNAM, publicado en Veterinaria México OA, y cosechado de Revistas UNAM

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Procedencia del contenido

Entidad o dependencia
Facultad de Medicina Veterinaria y Zootecnia, UNAM
Revista
Repositorio
Contacto
Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

Cita

Mycobacterium avium subsp. paratuberculosis down-regulates mRNA expression of iron-induced macrophage Ferroportin 1. (2016). Veterinaria México OA; Vol 3, No 1, 2016. Recuperado de https://repositorio.unam.mx/contenidos/60589

Descripción del recurso

Colaborador(es)
Landeros-sánchez, Bertha ; Díaz-aparicio, Efrén ; Gutierrez-pabello, Jose Angel ; Oshima, Sawako ; Medina-basulto, Gerardo Enrique ; Renteria-evangelista, Tomas Benjamin
Afiliación del colaborador
Instituto de Investigaciones Forestales, Agrícolas y Pecuarias, Mexico; Universidad Autónoma de Baja California; Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California; Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México
Tipo
Artículo de Investigación
Área del conocimiento
Biotecnología y Ciencias Agropecuarias
Título
Mycobacterium avium subsp. paratuberculosis down-regulates mRNA expression of iron-induced macrophage Ferroportin 1
Fecha
2016-03-31
Resumen
Veterinaria México OA ISSN: 2448-6760 Cite this as: • Landeros Sánchez B, Gutierrez Pabello JA, Medina Basulto GE, Renteria Evangelista TB, Díaz Aparicio E, Oshima S. Mycobacterium avium subsp. paratuberculosis down-regulates mRNA expression of iron-induced macrophage Ferroportin 1. Veterinaria México OA. 2016;3(1). doi: 10.21753/vmoa.3.1.361 Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease. The mechanisms by which MAP is able to adapt to the innate host response are still unclear. We examined Ferroportin 1 (FPN1) mRNA expression levels via real-time PCR of the mouse macrophage cell line J774 that was incubated in the presence of Mycobacterium avium subsp. paratuberculosis (MAP) or MAP crude protein extract. Infection with live MAP decreased FPN1 mRNA levels in a multiplicity of infection (MOI)-dependent fashion. Macrophages infected with MOIs of 20:1 and 15:1 did not show any change in FPN1 gene expression, whereas MOIs of 10:1 and 5:1 induced a decrease of 50 and 80%, respectively. Macrophages treated with 50, 100, 150 and 200 µg/mL of MAP crude extract (ATCC19698) decreased FPN1 mRNA expression by 25%. Additionally, up-regulation of FPN1 mRNA by an iron overload treatment of 400 µM of ferric nitrilotriacetate (FeNTA) was abrogated by live MAP (MOI 20:1) by approximately 70%. Our data revealed an inhibitory effect of MAP on FPN1 mRNA and suggested a bacterial mechanism that may play a role in host iron regulation. Figure 1. Infection by Mycobacterium avium subsp. paratuberculosis (MAP) down-regulates macrophage fpn1 gene expression. Murine macrophages, J774, were infected with MAP at different multiplicities of infection (MOIs) for 6 hours. Total RNA was extracted and gene expression was measured by real-time PCR. Results were normalized to GAPDH and expressed as units of relative expression (URE). Results are the mean ± standard deviation of 3 independent experiments with 3 replicates each. Statistical significance, P = 0.05.
Tema
Gene expression; Johne’s disease; Ferroportin 1; Iron; Macrophages
Idioma
eng
ISSN
2448-6760

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