dor_id: 4129963

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336.#.#.3: Artículo de Investigación

336.#.#.a: Artículo

351.#.#.6: https://jart.icat.unam.mx/index.php/jart

351.#.#.b: Journal of Applied Research and Technology

351.#.#.a: Artículos

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856.4.0.u: https://jart.icat.unam.mx/index.php/jart/article/view/1693/817

100.1.#.a: Domínguez, G.; Cardiel, E.; Reyes, J.L; Sánchez, E.; Hernández, P.R.

524.#.#.a: Domínguez, G., et al. (2021). Electrical impedance measurement system to assess in-situ experiments on epithelia under ELF magnetic fields exposure. Journal of Applied Research and Technology; Vol. 19 Núm. 3, 2021; 217-226. Recuperado de https://repositorio.unam.mx/contenidos/4129963

245.1.0.a: Electrical impedance measurement system to assess in-situ experiments on epithelia under ELF magnetic fields exposure

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Instituto de Ciencias Aplicadas y Tecnología, UNAM

264.#.0.c: 2021

264.#.1.c: 2021-06-30

653.#.#.a: Transepithelial electrical resistance measurement; electrical impedance of cells; Nonlinear Least Square Approximation Method; in situ experiments on epithelia; ELF MF exposure on epithelia

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC-SA 4.0 Internacional, https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.es, para un uso diferente consultar al responsable jurídico del repositorio por medio del correo electrónico gabriel.ascanio@icat.unam.mx

884.#.#.k: https://jart.icat.unam.mx/index.php/jart/article/view/1693

001.#.#.#: 074.oai:ojs2.localhost:article/1693

041.#.7.h: eng

520.3.#.a: Purpose The development of an electric impedance meter based on the impedance spectroscopy technique, for in vitro and in situ experimentation, with cellular epithelia submitted to extremely low frequency magnetic fields in a controlled environment. Unlike other reported systems, a strength of the one presented here is that it avoids the influence of external factors on the experiment. Materials and methods The designed system employs the electrical impedance values obtained by the impedance spectroscopy technique to determine the parameters of the simple equivalent electrical model of a cellular monolayer. The Madin-Darby Canine Kidney (MDCK) cell cultures were used as subjects of study in the experimental protocol. Results The validation was carried out by comparing the transepithelial electrical impedance data of the cell cultures obtained with the developed system and those of the Cellzscope® commercial system used as the standard. Non-significant differences were obtained. Conclusion It was confirmed that the developed system provides reliable values of transepithelial electrical impedance to experiment with cell cultures and take advantage of the controlled environment to reduce the effects of experimental management.

773.1.#.t: Journal of Applied Research and Technology; Vol. 19 Núm. 3 (2021); 217-226

773.1.#.o: https://jart.icat.unam.mx/index.php/jart

022.#.#.a: ISSN electrónico: 2448-6736; ISSN: 1665-6423

310.#.#.a: Bimestral

300.#.#.a: Páginas: 217-226

264.#.1.b: Instituto de Ciencias Aplicadas y Tecnología, UNAM

doi: https://doi.org/10.22201/icat.24486736e.2021.19.3.1693

harvesting_date: 2023-11-08 13:10:00.0

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file_creation_date: 2021-06-20 23:54:26.0

file_modification_date: 2021-06-20 23:54:26.0

file_creator: Yolanda G.G.

file_name: 619ecc5d365179186ce004e971846853b2ddb9dae44a8fbac90de665f6bbc486.pdf

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last_modified: 2024-03-19 14:00:00

license_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.es

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Artículo

Electrical impedance measurement system to assess in-situ experiments on epithelia under ELF magnetic fields exposure

Domínguez, G.; Cardiel, E.; Reyes, J.L; Sánchez, E.; Hernández, P.R.

Instituto de Ciencias Aplicadas y Tecnología, UNAM, publicado en Journal of Applied Research and Technology, y cosechado de Revistas UNAM

Licencia de uso

Procedencia del contenido

Cita

Domínguez, G., et al. (2021). Electrical impedance measurement system to assess in-situ experiments on epithelia under ELF magnetic fields exposure. Journal of Applied Research and Technology; Vol. 19 Núm. 3, 2021; 217-226. Recuperado de https://repositorio.unam.mx/contenidos/4129963

Descripción del recurso

Autor(es)
Domínguez, G.; Cardiel, E.; Reyes, J.L; Sánchez, E.; Hernández, P.R.
Tipo
Artículo de Investigación
Área del conocimiento
Ingenierías
Título
Electrical impedance measurement system to assess in-situ experiments on epithelia under ELF magnetic fields exposure
Fecha
2021-06-30
Resumen
Purpose The development of an electric impedance meter based on the impedance spectroscopy technique, for in vitro and in situ experimentation, with cellular epithelia submitted to extremely low frequency magnetic fields in a controlled environment. Unlike other reported systems, a strength of the one presented here is that it avoids the influence of external factors on the experiment. Materials and methods The designed system employs the electrical impedance values obtained by the impedance spectroscopy technique to determine the parameters of the simple equivalent electrical model of a cellular monolayer. The Madin-Darby Canine Kidney (MDCK) cell cultures were used as subjects of study in the experimental protocol. Results The validation was carried out by comparing the transepithelial electrical impedance data of the cell cultures obtained with the developed system and those of the Cellzscope® commercial system used as the standard. Non-significant differences were obtained. Conclusion It was confirmed that the developed system provides reliable values of transepithelial electrical impedance to experiment with cell cultures and take advantage of the controlled environment to reduce the effects of experimental management.
Tema
Transepithelial electrical resistance measurement; electrical impedance of cells; Nonlinear Least Square Approximation Method; in situ experiments on epithelia; ELF MF exposure on epithelia
Idioma
eng
ISSN
ISSN electrónico: 2448-6736; ISSN: 1665-6423

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