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Ver términos de la licenciaSandoval Larios, Giovanna, et al. (2024). El fucoidan de Sargassum sinicola es reconocido in vitro por la bacteria Campylobacter jejuni. TIP Revista Especializada en Ciencias Químico-Biológicas; Vol. 27, 2024. Recuperado de https://repositorio.unam.mx/contenidos/4161115
Autor(es)
Sandoval Larios, Giovanna; Palafox Félix, Sharon; Armenta Corral, Rosa Idalia; García-Hernández, Jaqueline; Balandrán-Quintana, René Renato; Clamont Montfort, Gabriela Ramos
Tipo
Artículo de Investigación
Área del conocimiento
Biología y Química
Título
El fucoidan de Sargassum sinicola es reconocido in vitro por la bacteria Campylobacter jejuni
Fecha
2024-12-03
Resumen
Campylobacteriosis is a significant global public health issue that requires new methods for preventing and treating the infection. One promising approach is to prevent the adhesion of Campylobacter jejuni to intestinal cells by blocking its binding sites with molecules that mimic the fucosylated receptors it recognizes. In this study, the fucosylated polysaccharide fucoidan was isolated from the brown algae Sargassum sinicola. The fucoidan was characterized, and its recognition by Campylobacter jejuni was tested in vitro. Fucoidan was extracted using aqueous methods at various temperatures, followed by selective precipitation with ethanol, trichloroacetic acid, calcium chloride, and additional ethanol. The yield of fucoidan was found to be 3.1 ± 0.29%, with 16.8 ± 0.46% fucose, 14.2 ± 1.2% sulfates, and 6.8 ± 0.02% uronic acids. The structural characteristics of the fucoidan were analyzed using Fourier transform infrared spectroscopy, which revealed the characteristic signals of carbohydrates and sulfate groups. The extracted fucoidan showed a Zeta potential of -18.5 ± 0.10 mV, a particle size of 127.3 ± 34.7 nm, and a Polydispersity Index of 0.80 ± 0.21. In vitro experiments demonstrated that Campylobacter jejuni recognized the extracted fucoidan, indicating its potential as a glycomimetic antagonist for this bacterium, which is currently the leading cause of infectious bacterial gastroenteritis worldwide.
Tema
brown seaweeds; fucoidan; glycomimetic antagonist; infection; algas pardas; fucoidan; antagonistas glicomiméticos; infección
Idioma
spa
ISSN
ISSN electrónico: 2395-8723; ISSN impreso: 1405-888X
