dor_id: 4149943

506.#.#.a: Público

590.#.#.d: Los artículos enviados a la revista "Veterinaria México OA", se juzgan por medio de un proceso de revisión por pares

510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT); Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex); Scientific Electronic Library Online (SciELO); Bibliografía Latinoamericana (Biblat); La Red de Revistas Científicas de América Latina y el Caribe, España y Portugal (Redalyc); Connecting research and researchers (ORCiD)

561.#.#.u: https://www.fmvz.unam.mx/

650.#.4.x: Biotecnología y Ciencias Agropecuarias

336.#.#.b: article

336.#.#.3: Artículo de Investigación

336.#.#.a: Artículo

351.#.#.6: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index

351.#.#.b: Veterinaria México OA

351.#.#.a: Artículos

harvesting_group: RevistasUNAM

270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

590.#.#.c: Open Journal Systems (OJS)

270.#.#.d: MX

270.1.#.d: México

590.#.#.b: Concentrador

883.#.#.u: https://revistas.unam.mx/catalogo/

883.#.#.a: Revistas UNAM

590.#.#.a: Coordinación de Difusión Cultural

883.#.#.1: https://www.publicaciones.unam.mx/

883.#.#.q: Dirección General de Publicaciones y Fomento Editorial

850.#.#.a: Universidad Nacional Autónoma de México

856.4.0.u: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/1022/925

100.1.#.a: Castellanos Zacarías, Carlos Adelso; Ramón Ugalde, Julio Porfirio; Loeza Concha, Henry Jesús; Zamora Bustillos, Roberto; Pinzón López, Luis Leonardo; Rodríguez Gutiérrez, Guadalupe Itzel; Domínguez Rebolledo, Álvaro Efrén

524.#.#.a: Castellanos Zacarías, Carlos Adelso, et al. (2023). Effect of two animal protein-free extenders on cryopreservation of Pelibuey and Blackbelly ram semen. Veterinaria México OA; Vol. 10, 2023. Recuperado de https://repositorio.unam.mx/contenidos/4149943

245.1.0.a: Effect of two animal protein-free extenders on cryopreservation of Pelibuey and Blackbelly ram semen

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Facultad de Medicina Veterinaria y Zootecnia, UNAM

264.#.0.c: 2023

264.#.1.c: 2023-06-26

653.#.#.a: semen; extender; ram; protein-free; cryopreservation; Reproductive biotechnology in small ruminants

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY 4.0 Internacional, https://creativecommons.org/licenses/by/4.0/legalcode.es, para un uso diferente consultar al responsable jurídico del repositorio por medio del correo electrónico vetmexicooa@gmail.com

884.#.#.k: https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/1022

001.#.#.#: 131.oai:ojs.pkp.sfu.ca:article/1022

041.#.7.h: eng

520.3.#.a: The study evaluated the effect of two animal protein-free extenders on ram semen cryopreservation of two tropical hair breeds. The ejaculates were collected from 8 rams (4 blackbelly and 4 pelibuey), which were mixed (pooled) by breed, diluted and cryopreserved in three different extenders animal protein [tris egg yolk (triladyl®)], and animal protein-free extenders andromed® (lecithin soy bean) and optixcell® (liposome). sperm analyses of total (tm) and progressive (pm) motility, viability, mitochondrial activity, acrosome integrity, and plasma membrane integrity (pmi) were carried out at 0 and 6 h after semen thawing. optixcell® and triladyl® extenders showed similar results between them and differed with andromed® in tm, pm, viability, and pmi (p<0.0017). in the blackbelly breed, the tm was higher (p<0.0159) in optixcell® than in andromed®. in the pelibuey breed, the optixcell® and triladyl® showed similar results between them and differed with andromed® in tm, pm, viability, mitochondrial activity, and pmi (p<0.0140). however, triladyl® showed a higher percentage of sperm with intact acrosome than andromed® (p<0.0392). in both breeds, spermatic parameters decreased progressively over the incubation time similarly in all three extenders. in conclusion, optixcell® and triladyl® proved to be the best extenders to cryopreserve blackbelly and pelibuey ram semen. however, optixcell® is an animal protein-free extender that decreases the risk of bacterial contamination, whereas triladyl® is composed of animal protein (egg yolk), which may impact the fertilizing capacity of sperm.

773.1.#.t: Veterinaria México OA; Vol. 10 (2023)

773.1.#.o: https://veterinariamexico.fmvz.unam.mx/index.php/vet/index

022.#.#.a: ISSN electrónico: 2448-6760

310.#.#.a: Trimestral

264.#.1.b: Facultad de Medicina Veterinaria y Zootecnia, UNAM

doi: https://doi.org/10.22201/fmvz.24486760e.2023.1022

harvesting_date: 2023-11-08 13:10:00.0

856.#.0.q: application/pdf

file_creation_date: 2023-06-26 22:58:29.0

file_modification_date: 2023-06-26 22:58:37.0

file_name: 3e2b79fc3165441d15448a3c909b41e3b95b1f680e50809a38387e9f94b9b847.pdf

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last_modified: 2024-03-19 14:00:00

license_url: https://creativecommons.org/licenses/by/4.0/legalcode.es

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Artículo

Effect of two animal protein-free extenders on cryopreservation of Pelibuey and Blackbelly ram semen

Castellanos Zacarías, Carlos Adelso; Ramón Ugalde, Julio Porfirio; Loeza Concha, Henry Jesús; Zamora Bustillos, Roberto; Pinzón López, Luis Leonardo; Rodríguez Gutiérrez, Guadalupe Itzel; Domínguez Rebolledo, Álvaro Efrén

Facultad de Medicina Veterinaria y Zootecnia, UNAM, publicado en Veterinaria México OA, y cosechado de Revistas UNAM

Licencia de uso

Procedencia del contenido

Entidad o dependencia
Facultad de Medicina Veterinaria y Zootecnia, UNAM
Revista
Repositorio
Contacto
Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

Cita

Castellanos Zacarías, Carlos Adelso, et al. (2023). Effect of two animal protein-free extenders on cryopreservation of Pelibuey and Blackbelly ram semen. Veterinaria México OA; Vol. 10, 2023. Recuperado de https://repositorio.unam.mx/contenidos/4149943

Descripción del recurso

Autor(es)
Castellanos Zacarías, Carlos Adelso; Ramón Ugalde, Julio Porfirio; Loeza Concha, Henry Jesús; Zamora Bustillos, Roberto; Pinzón López, Luis Leonardo; Rodríguez Gutiérrez, Guadalupe Itzel; Domínguez Rebolledo, Álvaro Efrén
Tipo
Artículo de Investigación
Área del conocimiento
Biotecnología y Ciencias Agropecuarias
Título
Effect of two animal protein-free extenders on cryopreservation of Pelibuey and Blackbelly ram semen
Fecha
2023-06-26
Resumen
The study evaluated the effect of two animal protein-free extenders on ram semen cryopreservation of two tropical hair breeds. The ejaculates were collected from 8 rams (4 blackbelly and 4 pelibuey), which were mixed (pooled) by breed, diluted and cryopreserved in three different extenders animal protein [tris egg yolk (triladyl®)], and animal protein-free extenders andromed® (lecithin soy bean) and optixcell® (liposome). sperm analyses of total (tm) and progressive (pm) motility, viability, mitochondrial activity, acrosome integrity, and plasma membrane integrity (pmi) were carried out at 0 and 6 h after semen thawing. optixcell® and triladyl® extenders showed similar results between them and differed with andromed® in tm, pm, viability, and pmi (p<0.0017). in the blackbelly breed, the tm was higher (p<0.0159) in optixcell® than in andromed®. in the pelibuey breed, the optixcell® and triladyl® showed similar results between them and differed with andromed® in tm, pm, viability, mitochondrial activity, and pmi (p<0.0140). however, triladyl® showed a higher percentage of sperm with intact acrosome than andromed® (p<0.0392). in both breeds, spermatic parameters decreased progressively over the incubation time similarly in all three extenders. in conclusion, optixcell® and triladyl® proved to be the best extenders to cryopreserve blackbelly and pelibuey ram semen. however, optixcell® is an animal protein-free extender that decreases the risk of bacterial contamination, whereas triladyl® is composed of animal protein (egg yolk), which may impact the fertilizing capacity of sperm.
Tema
semen; extender; ram; protein-free; cryopreservation; Reproductive biotechnology in small ruminants
Idioma
eng
ISSN
ISSN electrónico: 2448-6760

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