Artículo

COMPARISON OF TWO BIOMODELS (BALB/C MICE AND SPRAGUE DAWLEY RATS) IN THE ALKALINE COMET ASSAY

Arencibia Arrebola, Daniel Francisco; Rosario Fernández, Luis Alfredo; Suárez Fernández, Yolanda Emilia; Vidal Novoa, Alexis

Facultad de Estudios Superiores Iztacala, UNAM, publicado en BIOCYT. Biología, Ciencia y Tecnología ( (Revistas UNAM)

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La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC-ND 4.0 Internacional, https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es, fecha de asignación de la licencia no disponible, para un uso diferente consultar al responsable jurídico del repositorio por medio del correo electrónico hvazquez@unam.mx. Ver términos de la licencia

Procedencia del contenido

Cita

Arencibia Arrebola, Daniel Francisco, et al. (2011). COMPARISON OF TWO BIOMODELS (BALB/C MICE AND SPRAGUE DAWLEY RATS) IN THE ALKALINE COMET ASSAY. BIOCYT Biología Ciencia y Tecnología; Vol. 4 Núm. 13-16, 2011. Recuperado de https://repositorio.unam.mx/contenidos/4149218

Descripción del recurso

Autor(es)
Arencibia Arrebola, Daniel Francisco; Rosario Fernández, Luis Alfredo; Suárez Fernández, Yolanda Emilia; Vidal Novoa, Alexis
Tipo
Artículo de Investigación
Área del conocimiento
Biología y Química
Título
COMPARISON OF TWO BIOMODELS (BALB/C MICE AND SPRAGUE DAWLEY RATS) IN THE ALKALINE COMET ASSAY
Fecha
2020-05-29
Resumen
This article had as objective to carry out a comparison between Balb/c mice and Sprague Dawley rats as biomodel in the alkaline comet assay, keeping in mind the basal frequency and that induced with Cyclophosphamide, the induction of single strand breaks (SSB) or alkali-labile sites formation on DNA of peripheral blood leukocytes. Ten animal/sex/species/group of Balb/c mice and Sprague Dawley rats were used, and treated for 14 days. Using a negative control group (not treated), two substance-vehicle controls and a positive control received cyclophosphamide 50 mg/kg, via intraperitoneal. After two weeks the alkaline electrophoresis gel was performed for individual cells from leukocytes of peripheral blood to screen for possible DNA damage. The best biomodel in both sexes was the SD rats differing significantly with the results obtained in Balb/c mice keeping in mind the spontaneous and induced values of DNA damage. This study shows SD rats are a more efficient model for preclinical genotoxic evaluation of drugs, vaccines and natural products.
Tema
Comet Assay; Breaks; Single Strand; Labile Sites; Alkali; Dna; Balb/c Mice; Sprague Dawley Rats
Idioma
spa
ISSN
ISSN electrónico: 2007-2082
DOI
https://doi.org/10.22201/fesi.20072082.2011.4.75943

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