dor_id: 4149176

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856.4.0.u: https://www.revistascca.unam.mx/rica/index.php/rica/article/view/54614/47208

100.1.#.a: Cai, Sheng; Yang, Ping; Xu, Chen; Cai, Xiaoning

524.#.#.a: Cai, Sheng, et al. (2023). Cloning and analysis of the sequences of the super-selenium-rich plant Cardamine hupingshanensis. Revista Internacional de Contaminación Ambiental; Vol. 38, 2023: Environmental Science and Policy for Sustainable Development; 63-68. Recuperado de https://repositorio.unam.mx/contenidos/4149176

245.1.0.a: Cloning and analysis of the sequences of the super-selenium-rich plant Cardamine hupingshanensis

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Instituto de Ciencias de la Atmósfera y Cambio Climático, UNAM

264.#.0.c: 2023

264.#.1.c: 2023-02-24

653.#.#.a: Internal transcribed spacer; genetic; evolution; hypothesis; Internal transcribed spacer; genetic; evolution; hypothesis

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC 4.0 Internacional, https://creativecommons.org/licenses/by-nc/4.0/legalcode.es, para un uso diferente consultar al responsable jurídico del repositorio por medio del correo electrónico claudio.amescua@atmosfera.unam.mx

884.#.#.k: https://www.revistascca.unam.mx/rica/index.php/rica/article/view/54614

001.#.#.#: 105.oai:ojs.pkp.sfu.ca:article/54614

041.#.7.h: spa

520.3.#.a: We employed a pair of universal primers for PCR amplification of Cardamine hupingshanensis leaf DNA collected from the soil of a unique selenium-rich deposit in Yutangba, Enshi, China. Then the PCR products were sequenced to obtain the 852-bp DNA sequences containing internal transcribed spacers (ITS). The ITS DNA sequences of different Cardamine species were aligned and spliced to obtain a “reference” sequence. Next, the base differences were compared between the ITS sequences of different Cardamine species and the “reference” sequence. Our results showed that Cardamine hupingshanensis had the greatest differences (17) compared with the “reference” sequence, much more than those between the ITS sequences of other Cardamine species and the “reference” sequence and 3.2 times higher than the average. It is speculated that some Cardamine species growing in selenium-rich regions of Enshi for a long time produce genetic variations that can adapt to high selenium soil stress and develop a super selenium enrichment capacity, thus evolving the new species Cardamine hupingshanensis. Therefore, we proposed the hypothesis of accelerated evolution of Cardamine hupingshanensis under high selenium soil stress.

773.1.#.t: Revista Internacional de Contaminación Ambiental; Vol. 38 (2023): Environmental Science and Policy for Sustainable Development; 63-68

773.1.#.o: https://www.revistascca.unam.mx/rica/index.php/rica/index

022.#.#.a: ISSN impreso: 0188-4999

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300.#.#.a: Páginas: 63-68

264.#.1.b: Instituto de Ciencias de la Atmósfera y Cambio Climático, UNAM

doi: https://doi.org/10.20937/RICA.54614

harvesting_date: 2023-11-08 13:10:00.0

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file_creation_date: 2023-02-24 13:44:08.0

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245.1.0.b: Cloning and analysis of the sequences of the super-selenium-rich plant Cardamine hupingshanensis

last_modified: 2024-03-19 14:00:00

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Artículo

Cloning and analysis of the sequences of the super-selenium-rich plant Cardamine hupingshanensis

Cai, Sheng; Yang, Ping; Xu, Chen; Cai, Xiaoning

Instituto de Ciencias de la Atmósfera y Cambio Climático, UNAM, publicado en Revista Internacional de Contaminación Ambiental, y cosechado de Revistas UNAM

Licencia de uso

Procedencia del contenido

Cita

Cai, Sheng, et al. (2023). Cloning and analysis of the sequences of the super-selenium-rich plant Cardamine hupingshanensis. Revista Internacional de Contaminación Ambiental; Vol. 38, 2023: Environmental Science and Policy for Sustainable Development; 63-68. Recuperado de https://repositorio.unam.mx/contenidos/4149176

Descripción del recurso

Autor(es)
Cai, Sheng; Yang, Ping; Xu, Chen; Cai, Xiaoning
Tipo
Artículo de Investigación
Área del conocimiento
Biología y Química
Título
Cloning and analysis of the sequences of the super-selenium-rich plant Cardamine hupingshanensis
Fecha
2023-02-24
Resumen
We employed a pair of universal primers for PCR amplification of Cardamine hupingshanensis leaf DNA collected from the soil of a unique selenium-rich deposit in Yutangba, Enshi, China. Then the PCR products were sequenced to obtain the 852-bp DNA sequences containing internal transcribed spacers (ITS). The ITS DNA sequences of different Cardamine species were aligned and spliced to obtain a “reference” sequence. Next, the base differences were compared between the ITS sequences of different Cardamine species and the “reference” sequence. Our results showed that Cardamine hupingshanensis had the greatest differences (17) compared with the “reference” sequence, much more than those between the ITS sequences of other Cardamine species and the “reference” sequence and 3.2 times higher than the average. It is speculated that some Cardamine species growing in selenium-rich regions of Enshi for a long time produce genetic variations that can adapt to high selenium soil stress and develop a super selenium enrichment capacity, thus evolving the new species Cardamine hupingshanensis. Therefore, we proposed the hypothesis of accelerated evolution of Cardamine hupingshanensis under high selenium soil stress.
Tema
Internal transcribed spacer; genetic; evolution; hypothesis; Internal transcribed spacer; genetic; evolution; hypothesis
Idioma
spa
ISSN
ISSN impreso: 0188-4999

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