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Centro de Investigaciones Interdisciplinarias en Ciencias y Humanidades, UNAM; Instituto de Ciencias Aplicadas y Tecnología, UNAM; Centro de Nanociencias y Nanotecnología, UNAM
Revista
Repositorio
Contacto
Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx
Sánchez-Escamilla, José Luis, et al. (2025). Caracterización de osteoesferoides para inducir mineralización. Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología; Vol. 18 Núm. 34, 2025: Bionanomateriales. Recuperado de https://repositorio.unam.mx/contenidos/4160953
Autor(es)
Sánchez-Escamilla, José Luis; Valencia-González, Heriberto Abraham; Serrano-Bello, Janeth; Vázquez-Vázquez, Febe Carolina; Gaitán-Salvatella, Íñigo; Álvarez-Pérez, Marco Antonio; Maldonado-Frías, Silvia
Tipo
Artículo de Investigación
Área del conocimiento
Multidisciplina
Título
Caracterización de osteoesferoides para inducir mineralización
Fecha
2024-09-11
Resumen
This work shows the obtaining of osteospheroids, which have the ability to produce mineralized nodules, which can be used as study models to test nanodrugs or nanomaterials. Cell suspension cultures were made to obtain fetal osteoblast spheroids, with different cell concentrations/mL for 5 days. The condition where the diameter ranges from 80 to 150 μM was selected to make tests at 3, 7, 14 and 21 days. The cell viability of the osteospheroids was quantified by trypan blue exclusion and clonogenic assays were realized to determine the effect of the mineralizing medium on the formation of these structures. The integrity of the osteospheroids was observed by H&E and the formation of mineralized nodules was detected by alizarin red staining. The results show regular osteospheroids without aggregates at a concentration of 5 x 102 cells/mL and viable above 70% after 7 days of formation. Clonogenic assays do not show significant differences in morphology or the number of colonies between the control and the medium that induces mineralization. H&E stains show nuclei and defined cytoplasm at 3, 7, and 14 days, and at 7 and 14 days, alizarin red staining suggests that they are generating calcium deposits.
Tema
Osteospheroids; 3D culture; mineralized nodules; stem cell; osteoesferoides; cultivo tridimensional; nódulos mineralizados; células madre
Idioma
spa
ISSN
ISSN electrónico: 2448-5691; ISSN impreso: 2007-5979
