dor_id: 4128923

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336.#.#.b: article

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856.4.0.u: https://www.revistascca.unam.mx/rica/index.php/rica/article/view/54462/47160

100.1.#.a: Heras Martínez, Héctor Mario; Muñoz Castellanos, Laila Nayzzel; Bugarín, Alejandro; Ramos Sánchez, Víctor Hugo; Salmerón Ochoa, Iván; Chávez Flores, David

524.#.#.a: Heras Martínez, Héctor Mario, et al. (2022). Biodegradation of polycyclic aromatic hydrocarbons by Acremonium sp. activity. Revista Internacional de Contaminación Ambiental; Vol. 38, 2022; 261-269. Recuperado de https://repositorio.unam.mx/contenidos/4128923

245.1.0.a: Biodegradation of polycyclic aromatic hydrocarbons by Acremonium sp. activity

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Instituto de Ciencias de la Atmósfera y Cambio Climático, UNAM

264.#.0.c: 2022

264.#.1.c: 2022-12-15

653.#.#.a: PAHs; bioremediation; biodegradation; UHPLC; fungal degradation; PAHs; bioremediation; biodegradation; UHPLC; fungal degradation

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884.#.#.k: https://www.revistascca.unam.mx/rica/index.php/rica/article/view/54462

001.#.#.#: 105.oai:ojs.pkp.sfu.ca:article/54462

041.#.7.h: spa

520.3.#.a: Chihuahua State is the largest apple producer in Mexico. To prevent apples from freezing during the winter, petroleum-based fuels are used to heat the orchards. In this research work, a microorganism isolated from inside of a fuel plastic container was identified through morphological analysis as the fungus Acremonium sp. By cultivating the fungus in potato dextrose agar medium (PDA), we were able to study the biodegradation rates of three polycyclic aromatic hydrocarbons (PAHs). The isolated microorganism was incubated at 28 ºC in M9 mineral liquid medium using phenanthrene, anthracene, and pyrene as the only carbon source at a concentration of 50 µg/mL. Degradation of PAHs was monitored using an environmentally friendly reverse phase ultra-high-performance liquid chromatography (UHPLC) system, with a C18 column, 30% water acidified at 0.1% with trifluoroacetic acid, and 70% acetonitrile as the mobile phase, 40 ºC column temperature, and UV detection at 254 nm. After 30 days of biodegradation (optimal time), the final mean concentration for individual degradation experiments were 2.51 µg/mL phenanthrene, 3.02 µg/mL anthracene and 5.53 µg/mL pyrene. Those final concentrations indicate degradation percentile of 95%, 94%, and 89%, respectively. Biodegradation experiments combining all three PAHs showed similarly high degradation percentiles of 93%, 94%, and 85% for phenanthrene, anthracene, and pyrene, respectively. The dry weight average at day 30 in the individual and combined degradation experiments was 2.34 mg/mg PAHs degraded.

773.1.#.t: Revista Internacional de Contaminación Ambiental; Vol. 38 (2022); 261-269

773.1.#.o: https://www.revistascca.unam.mx/rica/index.php/rica/index

022.#.#.a: ISSN impreso: 0188-4999

310.#.#.a: Trimestral

300.#.#.a: Páginas: 261-269

264.#.1.b: Instituto de Ciencias de la Atmósfera y Cambio Climático, UNAM

doi: https://doi.org/10.20937/RICA.54462

harvesting_date: 2023-11-08 13:10:00.0

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245.1.0.b: Biodegradation of polycyclic aromatic hydrocarbons by Acremonium sp. activity

last_modified: 2024-03-19 14:00:00

license_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode.es

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Artículo

Biodegradation of polycyclic aromatic hydrocarbons by Acremonium sp. activity

Heras Martínez, Héctor Mario; Muñoz Castellanos, Laila Nayzzel; Bugarín, Alejandro; Ramos Sánchez, Víctor Hugo; Salmerón Ochoa, Iván; Chávez Flores, David

Instituto de Ciencias de la Atmósfera y Cambio Climático, UNAM, publicado en Revista Internacional de Contaminación Ambiental, y cosechado de Revistas UNAM

Licencia de uso

Procedencia del contenido

Cita

Heras Martínez, Héctor Mario, et al. (2022). Biodegradation of polycyclic aromatic hydrocarbons by Acremonium sp. activity. Revista Internacional de Contaminación Ambiental; Vol. 38, 2022; 261-269. Recuperado de https://repositorio.unam.mx/contenidos/4128923

Descripción del recurso

Autor(es)
Heras Martínez, Héctor Mario; Muñoz Castellanos, Laila Nayzzel; Bugarín, Alejandro; Ramos Sánchez, Víctor Hugo; Salmerón Ochoa, Iván; Chávez Flores, David
Tipo
Artículo de Investigación
Área del conocimiento
Biología y Química
Título
Biodegradation of polycyclic aromatic hydrocarbons by Acremonium sp. activity
Fecha
2022-12-15
Resumen
Chihuahua State is the largest apple producer in Mexico. To prevent apples from freezing during the winter, petroleum-based fuels are used to heat the orchards. In this research work, a microorganism isolated from inside of a fuel plastic container was identified through morphological analysis as the fungus Acremonium sp. By cultivating the fungus in potato dextrose agar medium (PDA), we were able to study the biodegradation rates of three polycyclic aromatic hydrocarbons (PAHs). The isolated microorganism was incubated at 28 ºC in M9 mineral liquid medium using phenanthrene, anthracene, and pyrene as the only carbon source at a concentration of 50 µg/mL. Degradation of PAHs was monitored using an environmentally friendly reverse phase ultra-high-performance liquid chromatography (UHPLC) system, with a C18 column, 30% water acidified at 0.1% with trifluoroacetic acid, and 70% acetonitrile as the mobile phase, 40 ºC column temperature, and UV detection at 254 nm. After 30 days of biodegradation (optimal time), the final mean concentration for individual degradation experiments were 2.51 µg/mL phenanthrene, 3.02 µg/mL anthracene and 5.53 µg/mL pyrene. Those final concentrations indicate degradation percentile of 95%, 94%, and 89%, respectively. Biodegradation experiments combining all three PAHs showed similarly high degradation percentiles of 93%, 94%, and 85% for phenanthrene, anthracene, and pyrene, respectively. The dry weight average at day 30 in the individual and combined degradation experiments was 2.34 mg/mg PAHs degraded.
Tema
PAHs; bioremediation; biodegradation; UHPLC; fungal degradation; PAHs; bioremediation; biodegradation; UHPLC; fungal degradation
Idioma
spa
ISSN
ISSN impreso: 0188-4999

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