dor_id: 43364

506.#.#.a: Público

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510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT), Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex), Scientific Electronic Library Online (SciELO)

561.#.#.u: http://www.odonto.unam.mx/

561.#.#.a: Facultad de Odontología, UNAM

650.#.4.x: Medicina y Ciencias de la Salud

336.#.#.b: article

336.#.#.3: Artículo Técnico-Profesional

336.#.#.a: Artículo

351.#.#.6: http://revistas.unam.mx/index.php/rom

351.#.#.b: Revista Odontológica Mexicana

351.#.#.a: Artículos

harvesting_group: RevistasUNAM

270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

590.#.#.c: Open Journal Systems (OJS)

270.#.#.d: MX

270.1.#.d: México

590.#.#.b: Concentrador

883.#.#.u: http://www.revistas.unam.mx/front/

883.#.#.a: Revistas UNAM

590.#.#.a: Coordinación de Difusión Cultural, UNAM

883.#.#.1: https://www.publicaciones.unam.mx/

883.#.#.q: Dirección General de Publicaciones y Fomento Editorial, UNAM

850.#.#.a: Universidad Nacional Autónoma de México

856.4.0.u: http://revistas.unam.mx/index.php/rom/article/view/63578/55738

100.1.#.a: Albiero, Eduardo; Gobbi, Carla; Yorio, Marcelo; Busamia, Beatriz

524.#.#.a: Albiero, Eduardo, et al. (2018). Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients. Revista Odontológica Mexicana; Vol 22, No 1 Ing, 2018. Recuperado de https://repositorio.unam.mx/contenidos/43364

245.1.0.a: Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Facultad de Odontología, UNAM

264.#.0.c: 2018

264.#.1.c: 2018-02-28

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC-ND 4.0 Internacional, https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es, fecha de asignación de la licencia 2018-02-28, para un uso diferente consultar al responsable jurídico del repositorio por medio de danyelacarmona@gmail.com

884.#.#.k: http://revistas.unam.mx/index.php/rom/article/view/63578

041.#.7.h: eng

520.3.#.a: The aim of the present research project was to compare two techniques for oral cytology study (exfoliative cytology and impression cytology) in order to assess changes in oral mucosa which might allow non -invasive diagnosis of Sjögren’s syndrome cases (SS). Patients: 50 patients were selected, patients were paired by age and gender, and had been diagnosed with Sjögren’s syndrome according to criteria of the American-European Consensus. Patients were distributed into the following three experimental groups: Healthy control group (C), n = 14, Dry mouth and eyes group without SS, (ME) n = 13, and SS group n = 23. Material and methods: A cell harvesting brush (Cytobrush) was used for the exfoliative cytology procedure, sliding it along a glass plate and later fixating harvested cells in 95% ethanol. Cellulose acetate paper (Millipore Hawp 304®) was used for the impression cytology procedure. The paper was in 1 cm long stripes which were placed on the oral mucosa surface above upper vestibular groove; stripes were immobilized and pressure was applied for three seconds. Papanicolau (PAP) technique was used for dyeing. Morphology and histomorphology were assessed studying the following: cytoplasmic area (CA), nuclear areas (NA) nucleus-cytoplasm relationship (N:C) and amount of cells per square millimeter (mm2). Results: Both techniques revealed the following in C: isolated single-layered epithelial cells, basophils, normal central nuclei, 20 to 30 per mm2 , N:C ratio 1:8. In the eye and mouth group (EM): grouped and folded isolated cells, cytoplasm with eosinophilic predominance increase of cell amount to 40 per mm2 , nucleus-cytoplasm relationship N/C 1:4. SS patients showed the following: nuclear area with denser chromatin, 400 cells per mm2 , and 1:2 N/C relationship with respect to mouth and ears and control. Statistically significant differences wereobserved among groups in all studied characteristics. Conclusion: We can infer that impression cytology can be used in systemic and oral lesion’s diagnosis in patients afflicted with hyposalivation.

773.1.#.t: Revista Odontológica Mexicana; Vol 22, No 1 Ing (2018)

022.#.#.a: ISSN: 1870-199X

264.#.1.b: Facultad de Odontología, UNAM

758.#.#.1: http://revistas.unam.mx/index.php/rom/index

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harvesting_date: 2019-02-06 00:00:00.0

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Artículo

Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients

Albiero, Eduardo; Gobbi, Carla; Yorio, Marcelo; Busamia, Beatriz

Facultad de Odontología, UNAM, publicado en Revista Odontológica Mexicana, y cosechado de Revistas UNAM

Licencia de uso

Procedencia del contenido

Entidad o dependencia
Facultad de Odontología, UNAM
Revista
Revista Odontológica Mexicana
Repositorio
Revistas UNAM
Contacto
Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

Cita

Albiero, Eduardo, et al. (2018). Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients. Revista Odontológica Mexicana; Vol 22, No 1 Ing, 2018. Recuperado de https://repositorio.unam.mx/contenidos/43364

Descripción del recurso

Autor(es)
Albiero, Eduardo; Gobbi, Carla; Yorio, Marcelo; Busamia, Beatriz
Tipo
Artículo Técnico-Profesional
Área del conocimiento
Medicina y Ciencias de la Salud
Título
Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients
Fecha
2018-02-28
Resumen
The aim of the present research project was to compare two techniques for oral cytology study (exfoliative cytology and impression cytology) in order to assess changes in oral mucosa which might allow non -invasive diagnosis of Sjögren’s syndrome cases (SS). Patients: 50 patients were selected, patients were paired by age and gender, and had been diagnosed with Sjögren’s syndrome according to criteria of the American-European Consensus. Patients were distributed into the following three experimental groups: Healthy control group (C), n = 14, Dry mouth and eyes group without SS, (ME) n = 13, and SS group n = 23. Material and methods: A cell harvesting brush (Cytobrush) was used for the exfoliative cytology procedure, sliding it along a glass plate and later fixating harvested cells in 95% ethanol. Cellulose acetate paper (Millipore Hawp 304®) was used for the impression cytology procedure. The paper was in 1 cm long stripes which were placed on the oral mucosa surface above upper vestibular groove; stripes were immobilized and pressure was applied for three seconds. Papanicolau (PAP) technique was used for dyeing. Morphology and histomorphology were assessed studying the following: cytoplasmic area (CA), nuclear areas (NA) nucleus-cytoplasm relationship (N:C) and amount of cells per square millimeter (mm2). Results: Both techniques revealed the following in C: isolated single-layered epithelial cells, basophils, normal central nuclei, 20 to 30 per mm2 , N:C ratio 1:8. In the eye and mouth group (EM): grouped and folded isolated cells, cytoplasm with eosinophilic predominance increase of cell amount to 40 per mm2 , nucleus-cytoplasm relationship N/C 1:4. SS patients showed the following: nuclear area with denser chromatin, 400 cells per mm2 , and 1:2 N/C relationship with respect to mouth and ears and control. Statistically significant differences wereobserved among groups in all studied characteristics. Conclusion: We can infer that impression cytology can be used in systemic and oral lesion’s diagnosis in patients afflicted with hyposalivation.
Idioma
eng
ISSN
ISSN: 1870-199X

Enlaces