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Ver términos de la licenciaCastañeda-Roldán, Elsa I., et al. (2024). Aislamiento de Brucella melitensis en suelo agrícola, Puebla, México. Revista Internacional de Contaminación Ambiental; Vol. 40, 2024; 723-739. Recuperado de https://repositorio.unam.mx/contenidos/4161336
Autor(es)
Castañeda-Roldán, Elsa I.; Cruz-Aviña, Juan R.; Tenorio-Arvide, María G.; Valera-Pérez, Miguel A.; Villa-Mancera, Abel E.; Díaz-Larrea, Jhoana; Cabrera, Rubén
Tipo
Artículo de Investigación
Área del conocimiento
Biología y Química
Título
Aislamiento de Brucella melitensis en suelo agrícola, Puebla, México
Fecha
2024-11-21
Resumen
Brucellosis is an important zoonotic disease due to its global distribution, infectious-contagious potential (half a million new patients yearly), and a wide variety of niches (livestock, humans, wildlife, water, and air). A large part of these effects on the productive yields of cattle and goats in the Emilio Portes Gil community in the municipality of Puebla, Mexico, is attributed to Brucella. Therefore, this study aims to isolate Brucella sp. in agricultural soil samples in an area with a high incidence in animals and humans. Agricultural soil samples were used (n = 90). By standard microbiological methods, Brucella melitensis was isolated, and its presence was detected and confirmed in the analyzed samples, 100% for the primary isolation (total samples), 69.8% during reseeding, and 57% in the endpoint PCR confirmatory tests (using BM16 and E. coli as controls). The bp26 gene was amplified by 1024 bp, and the microbiological profiles were identical for B. melitensis. This is the first record of detection, isolation, and confirmation of B. melitensis in agricultural soil in Mexico. The soil system is recognized as an extension of the niche for B. melitensis. These data constitute a useful tool to improve understanding of the pathogenesis and virulence of the Brucella genus in a natural environment and its potential effect on public and animal health.
Tema
ground contamination; environmental zoonosis; endemic zone; contaminación de terreno; zoonosis ambiental; zona endémica
Idioma
spa
ISSN
ISSN impreso: 0188-4999
