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Ver términos de la licenciaMorales-Campos, Brenda Karina, et al. (2024). Actividad de celulasas y lacasas de Pleurotus ostreatus por fermentación en medios sólidos con subproductos del maíz (Zea mays L.). Revista Internacional de Contaminación Ambiental; Vol. 40, 2024; 331-340. Recuperado de https://repositorio.unam.mx/contenidos/4161042
Autor(es)
Morales-Campos, Brenda Karina; Sánchez-Santillán, Paulino; Torres-Salado, Nicolás; Saavedra-Jiménez, Luis Antonio; Herrera-Pérez, Jerónimo; Ayala-Monter, Marco Antonio
Tipo
Artículo de Investigación
Área del conocimiento
Biología y Química
Título
Actividad de celulasas y lacasas de Pleurotus ostreatus por fermentación en medios sólidos con subproductos del maíz (Zea mays L.)
Fecha
2024-11-21
Resumen
Maize by-products (corn stover, totomoxtle and stubble) could serve as a substrate to produce enzyme extracts by solid fermentation (SF). The objective of this study was to quantify the activity of cellulase and laccase enzymes of Pleurotus ostreatus strains at five and ten days of SF. The SF was carried out in Erlenmeyer flasks with 50 g of substrate (80% humidity) inoculated with 5 g of a strain and incubated at 30 ºC. At each fermentation time, the enzymatic extract was obtained and crude protein (CP), organic matter (OM), neutral detergent fiber (NDF) and acid detergent fiber (ADF) were determined from the solid ferment. The experimental design was a 2x3x2 factorial arrangement in a completely randomized design; using strains, substrates and fermentation time as factors. Cellulase enzyme activity and CP showed no differences between strains and substrate types (p > 0.05). The SF at ten days showed higher cellulase activity and CP than at five days; the highest laccase activity was in the SF for five days using strain P15 and corn stover or stubble; the ferment with stubble showed lower NDF than totomoxtle and corn stover; the solid ferment at ten days showed higher ADF than at five days of SF; the SF with ten days using stubble and strain MR showed lower OM (p < 0.05). In conclusion, olote and totomoxtle are effective substrates in SF to produce enzymatic extracts of cellulases and laccases, and the nutrient content of the substrate changed with fermentation time.
Tema
actividad enzimática; hongo podredumbre blanca; olote; tiempo de fermentación; totomoxtle; Enzyme activity; cob; fermentation time; totomoxtle; white rot fungi
Idioma
spa
ISSN
ISSN impreso: 0188-4999
