dor_id: 4109845

506.#.#.a: Público

590.#.#.d: El sistema de arbitraje es ciego; por pares anónimos, externos.

510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT), Directory of Open Access Journals (DOAJ), Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex), Scientific Electronic Library Online (SciELO), SCOPUS, Web Of Science (WoS)

561.#.#.u: http://www.ib.unam.mx/

650.#.4.x: Biología y Química

336.#.#.b: article

336.#.#.3: Artículo de Investigación

336.#.#.a: Artículo

351.#.#.6: http://www.revista.ib.unam.mx/index.php/bio

351.#.#.b: Revista Mexicana de Biodiversidad

351.#.#.a: Artículos

harvesting_group: RevistasUNAM

270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx

590.#.#.c: Open Journal Systems (OJS)

270.#.#.d: MX

270.1.#.d: México

590.#.#.b: Concentrador

883.#.#.u: http://www.revistas.unam.mx/front/

883.#.#.a: Revistas UNAM

590.#.#.a: Coordinación de Difusión Cultural

883.#.#.1: https://www.publicaciones.unam.mx/

883.#.#.q: Dirección General de Publicaciones y Fomento Editorial, UNAM

850.#.#.a: Universidad Nacional Autónoma de México

856.4.0.u: http://www.revista.ib.unam.mx/index.php/bio/article/view/1275/1786

100.1.#.a: González, Dolores; Vovides, Andrew P.

524.#.#.a: González, Dolores, et al. (2012). A modification to the SCAR (Sequence Characterized Amplified Region) method provides phylogenetic insights within Ceratozamia (Zamiaceae). Revista Mexicana de Biodiversidad; Vol. 83, núm. 4, 2012: diciembre. Recuperado de https://repositorio.unam.mx/contenidos/4109845

245.1.0.a: A modification to the SCAR (Sequence Characterized Amplified Region) method provides phylogenetic insights within Ceratozamia (Zamiaceae)

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Instituto de Biología, UNAM

264.#.0.c: 2012

264.#.1.c: 2012-07-01

653.#.#.a: Phylogeny; Cycads; Digested SCAR; molecular markers; RAPD; restriction enzymes; polymorphic loci; parsimony

506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC-ND 4.0 Internacional, https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es, fecha de asignación de la licencia 2012-07-01, para un uso diferente consultar al responsable jurídico del repositorio por medio de falvarez@ib.unam.mx

884.#.#.k: http://www.revista.ib.unam.mx/index.php/bio/article/view/1275

001.#.#.#: oai:ojs.132.248.13.2:article/1275

041.#.7.h: eng

520.3.#.a: Phylogenetic relationships among closely related plant species is still problematic. DNA intergenic regions often are insufficiently variable to provide desired resolution or support. In this study, a modification to the Sequence Characterized Amplified Region (SCAR) method was used to find polymorphic loci for phylogenetic analyses within Ceratozamia. RAPD markers were first used to detect variation in five species. Then, equal length fragments in two or more species were excised from the gel, purified and digested with frequent cutter restriction enzymes for isolating both ends, which have the same primer site. Later, digested fragments were sequenced with the RAPD primer. Last, variable sequences were used to design specific primers for amplifying and sequencing all species for phylogenetic analyses. Our results confirmed the previously known high genome sequence resemblance within this genus that contrasts with its high morphological variation. Only six parsimony informative characters were generated with this approach. Nonetheless, the SCAR method provided some phylogenetic insights. Three main clades consistent with distribution ranges of the species were detected. The approach presented here was effective to solve some relationships within the genus and can be implemented in other organisms to find polymorphic loci for phylogenetic studies.

773.1.#.t: Revista Mexicana de Biodiversidad; Vol. 83, núm. 4 (2012): diciembre

773.1.#.o: http://www.revista.ib.unam.mx/index.php/bio

046.#.#.j: 2021-02-09 00:00:00.000000

022.#.#.a: 2007-8706; 1870-3453

310.#.#.a: Trimestral

780.1.2.t: Anales del Instituto de Biología serie Botánica y Anales del Instituto de Biología serie Zoología

264.#.1.b: Instituto de Biología, UNAM

758.#.#.1: http://www.revista.ib.unam.mx/index.php/bio

doi: https://doi.org/10.22201/ib.20078706e.2012.4.1275

handle: 76bab55bcb856ae7

harvesting_date: 2020-09-23 00:00:00.0

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